The Definitive Answer: Serum First, Then Moisturizer. Always.
When building your nighttime skincare routine, the order you apply your products matters. The most common question is whether to apply serum or moisturizer first. The answer is clear and simple: you should always apply your serum before your moisturizer.
This rule is based on a simple principle: layer your products from the thinnest texture to the thickest. Serums are lightweight treatments designed to sink into the skin quickly. Moisturizers are richer and create a top layer to seal everything in. Applying them in this order ensures each product can do its job properly.
Think of your serum as the targeted treatment and your moisturizer as the protective blanket. The serum delivers potent ingredients, and the moisturizer locks them in while keeping your skin comfortable and hydrated. Following this order is a core part of our holistic approach to natural skincare, ensuring you get the most out of your evening ritual.
The 'Why': Understanding the Role of Serums vs. Moisturizers at Night
While both products care for your skin, they have different jobs, especially at night when your skin is in a natural state of renewal.
Serums Are Your 'Treatment' Step
Serums are lightweight, concentrated formulas. They are made of smaller molecules that can absorb quickly and deeply into the skin's surface. This makes them perfect for delivering a high dose of active ingredients that target specific concerns like uneven texture, dullness, or the appearance of fine lines.
A perfect example is the Empris-Peony™ Biotic Resurfacing Night Serum. This award-winning formula, named "Best Overnight Treatment" in the Cosmopolitan Clean Beauty Awards 2024, is designed to work while you sleep. It contains Niacinamide to support a balanced, even-looking complexion. It also uses gentle flower acids from Hibiscus Sabdariffa Flower Extract to exfoliate, revealing a smoother, more luminous appearance by morning.

Moisturizers Are Your 'Protector' Step
Moisturizers have a thicker consistency and larger molecules than serums. Their primary job is to hydrate the skin's surface and create a protective barrier. This barrier is crucial for two reasons: it locks in the serum you just applied, and it prevents moisture from escaping your skin overnight.
Using a moisturizer after your serum seals in its potent ingredients and supports your skin's moisture barrier.
Our Biotic Radiance-Boosting Hydrator is a silky, oil-free cream that feels both light and nourishing. It uses skin-improving bioferments like Lactobacillus Ferment Lysate to support a healthy skin microbiome. It also contains plant-derived Squalane, an exceptional hydrator that helps your skin feel soft, comfortable, and smooth without clogging pores.

What Happens If You Skip Moisturizer After Serum?
You might wonder if applying a moisturizer after your serum is truly necessary, especially if your skin feels hydrated from the serum alone. The answer is yes, it is a non-negotiable step for several important reasons.
First, you risk dehydration. Many serums, particularly those with humectants, draw moisture to the skin. Without a moisturizer to lock that moisture in, it can evaporate from your skin's surface, a process called Transepidermal Water Loss (TEWL). This can leave your skin feeling tighter and drier than before.
Second, your serum may not work as well. The active ingredients need to stay on your skin to be effective. Without a moisturizing layer to seal them in, some of the product can evaporate before it has a chance to be fully absorbed. This means you might not see the full benefits of your powerful treatment.
Finally, skipping this step can affect your skin's natural barrier. A healthy moisture barrier is key to comfortable, resilient-looking skin. When it's compromised, your skin may become more prone to the feeling of sensitivity and irritation. Our formulas use botanicals with soothing properties, such as Eclipta Prostrata Extract, to help keep skin feeling calm and comfortable.
Your Step-by-Step Nuebiome Night Ritual
Creating a consistent nighttime routine helps you get the most benefit from each product. This simple, four-step ritual ensures your skin is perfectly prepped for overnight renewal.
Cleanse: Start with a gentle cleanser, like our Biome Balancing Creamy Cleanser, to wash away makeup, sunscreen, and impurities without stripping your skin. Pat your skin dry with a soft towel.
Prep: Apply a few drops of The Biotic Glow™ Ferment Essence onto your palms and press it into your skin. This watery layer helps hydrate and prepare your skin to better absorb the products that follow.
Help care for: Apply one to two pumps of the Empris-Peony™ Biotic Resurfacing Night Serum. Gently massage it over your face and neck, avoiding the eye area. This is your power step to target specific skin concerns.
Seal & Moisturize: Finish by applying the Biotic Radiance-Boosting Hydrator. This final layer locks in the serum, supports your moisture barrier, and keeps your skin feeling comfortable all night long.
Pro-Tips: Elevating Your Nighttime Layering
Once you have mastered the basics, these professional tips can help you refine your routine even further.
Give It a Minute
Allow your serum to fully absorb before applying your moisturizer. A good rule of thumb is to wait about 60 seconds. This prevents the products from pilling (creating little balls on your skin) and ensures the serum has time to sink in.
Where Do Face Oils Fit In?
Face oils are a wonderful way to add extra nourishment, especially if your skin is dry or you live in a dry climate. Because oils are the thickest and most occlusive products, they should be applied last. After your moisturizer has absorbed, gently press a few drops of a face oil onto your skin. This creates the final seal, locking in both your serum and moisturizer.
The Skin Soothing Face Oil is a beautiful, lightweight option. It features cold-pressed Calophyllum Inophyllum (Tamanu) Oil and plant-derived Squalane to soothe and hydrate the skin, leaving behind a comfortable, velvety finish.

Layering Multiple Serums
If you use more than one serum, continue to follow the thin-to-thick rule. Start with the serum that has the most watery consistency and finish with the one that feels richer or more gel-like. To learn more, discover our full guide to unlocking the power of serums.
Discover Your Perfect Nighttime Pair
Building an effective nighttime routine doesn't have to be complicated. The simple, powerful combination of a targeted serum followed by a protective moisturizer is the key to waking up with skin that looks refreshed and feels comfortable.
By pairing the Empris-Peony™ Biotic Resurfacing Night Serum with the Biotic Radiance-Boosting Hydrator, you create a complete system for nighttime renewal. The serum works to refine and smooth your skin's appearance, while the hydrator seals in those benefits and supports your moisture barrier.
For those looking for a comprehensive, curated system, our Ultimate Microbiome Anti-Aging Routine includes everything you need for radiant-looking skin. You can also explore our complete collection of powerful face essences and serums to find the perfect solutions for your unique skin needs.
As with any new product, we always recommend patch testing first. If you have specific skin concerns or sensitivities, it's best to check with a licensed professional. For more answers to your skincare questions, please visit our FAQ page.
Further Reading
Our approach to formulation focuses on combining fermented ingredients with effective botanicals. Many of these botanicals have a long history of use in skincare. For example, some formulas use ingredients to help soothe and unify skin tone with antioxidant properties from botanicals like Curcuma Longa (Turmeric) Root Extract.
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